Absence of systemic immune response to adenovectors after intraocular administration to children with retinoblastoma.
Submitted by Cristhian J Ildefonso on
Title | Absence of systemic immune response to adenovectors after intraocular administration to children with retinoblastoma. |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Ildefonso, CJ, Kong, L, Leen, A, Chai, SJ, Petrochelli, V, Chintagumpala, M, Hurwitz, MY, Chévez-Barrios, P, Hurwitz, RL |
Journal | Mol Ther |
Volume | 18 |
Issue | 10 |
Pagination | 1885-90 |
Date Published | 2010 Oct |
ISSN | 1525-0024 |
Keywords | Adenoviridae, Antigen-Presenting Cells, Eye, Genetic Therapy, Genetic Vectors, Humans, Immunohistochemistry, Macrophages, Retinoblastoma, T-Lymphocytes, Thymidine Kinase |
Abstract | The ocular environment has been shown to induce tolerance to locally administered antigens. We therefore investigated whether there was a systemic immune response against adenoviral vectors injected into the vitreous of retinoblastoma patients enrolled in a phase 1 clinical trial of adenoviral-mediated thymidine kinase gene transfer. Sections of enucleated eyes were immunostained with antibodies against inflammatory cells. A trend toward increasing numbers of plasma cells, T cells, macrophages, and antigen-presenting cells was observed in the injected subjects' eyes, but systemically, there was no significant increase in the number of adenovirus-specific cytotoxic T lymphocytes (CTLs) or in adenovirus neutralizing antibodies. Therefore, in contrast to studies showing significant immunogenicity of Ad-RSVtk following injection into extraocular tumors, injection into the eye produces only a mild local inflammatory response without evidence of systemic cellular or humoral immune responses to adenovirus. |
DOI | 10.1038/mt.2010.139 |
Alternate Journal | Mol. Ther. |
PubMed ID | 20606647 |
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