A cell-based fluorescent assay to detect the activity of Shiga toxin and other toxins that inhibit protein synthesis
Submitted by Beatriz Quiñones on
PDF version| Title | A cell-based fluorescent assay to detect the activity of Shiga toxin and other toxins that inhibit protein synthesis |
| Publication Type | Journal Article |
| Year of Publication | 2011 |
| Authors | Massey, S, Quiñones, B, Teter, K |
| Journal | Methods Mol BiolMethods Mol BiolMethods Mol Biol |
| Volume | 739 |
| Pagination | 49-59 |
| ISBN Number | 1940-6029 (Electronic)1064-3745 (Linking) |
| Accession Number | 21567317 |
| Abstract | Escherichia coli O157:H7, a major cause of food-borne illness, produces Shiga toxins (Stxs) that block protein synthesis by inactivating the ribosome. In this chapter, we describe a simple cell-based fluorescent assay to detect Stxs and inhibitors of toxin activity. The assay can also be used to detect other plant and bacterial toxins that arrest protein synthesis. |
| Short Title | Methods in molecular biology (Clifton, N.JMethods in molecular biology (Clifton, N.J |
| Alternate Journal | Methods in molecular biology (Clifton, N.J |
