Characterization and differential expression of a ferritin protein from Fasciola hepatica.

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TitleCharacterization and differential expression of a ferritin protein from Fasciola hepatica.
Publication TypeJournal Article
Year of Publication2012
AuthorsCabán-Hernández, K, Gaudier, JF, Espino, AM
JournalMol Biochem Parasitol
Date Published2012 Mar-Apr
KeywordsAmino Acid Sequence, Animals, Antigens, Helminth, Biological Markers, Cloning, Molecular, DNA, Complementary, Escherichia coli, Fasciola hepatica, Fascioliasis, Ferritins, Gene Expression Regulation, Developmental, Helminth Proteins, Immunologic Tests, Iron, Molecular Sequence Data, Phylogeny, Protein Structure, Secondary, Rabbits, Recombinant Proteins, RNA, Messenger, Sequence Alignment

Ferritins are proteins that play a central role in maintaining intracellular iron balance. A cDNA clone of Fasciola hepatica (687 bp long) encoding a putative 228-amino acid polypeptide (FhFtn-1) homologous with ferritins of vertebrates and invertebrates was identified. FhFtn-1 contains a conserved motif of the ferroxidase center typical of vertebrate ferritins. Phylogenetic tree analysis showed that FhFtn-1 clusters with two ferritins of Paragonimus westermani, which suggests a common ancestry for the ferritins of these two trematodes. Recombinant FhFtn-1 protein expressed and purified from an Escherichia coli system showed iron-uptake ability. Moreover, FhFtn-1 showed strong reactivity with sera from rabbits infected with F. hepatica for 2-12 weeks, which suggests that this protein could be a potential antigen for immunodiagnosis of fascioliasis. qPCR analysis demonstrated that FhFtn-1-mRNA is expressed at significantly higher levels in adults and unembryonated eggs than in juveniles or miracidia. These results represent the first characterization of a ferritin protein from the liver fluke F. hepatica.

Alternate JournalMol. Biochem. Parasitol.
PubMed ID22240114
PubMed Central IDPMC3277670
Grant List1SC1AI096108-01A2 / AI / NIAID NIH HHS / United States
3 U54 RR022762-0351 / RR / NCRR NIH HHS / United States
R25GM061838 / GM / NIGMS NIH HHS / United States
SC1 AI096108 / AI / NIAID NIH HHS / United States
SC1 AI096108-01A2 / AI / NIAID NIH HHS / United States
U54 RR022762 / RR / NCRR NIH HHS / United States