Development of two antibody detection enzyme-linked immunosorbent assays for serodiagnosis of human chronic fascioliasis.

Kimberly Cabán-Hernández's picture
TitleDevelopment of two antibody detection enzyme-linked immunosorbent assays for serodiagnosis of human chronic fascioliasis.
Publication TypeJournal Article
Year of Publication2014
AuthorsCabán-Hernández, K, Gaudier, JF, Ruiz-Jiménez, C, Espino, AM
JournalJ Clin Microbiol
Date Published2014 Mar
KeywordsAdult, Animals, Antibodies, Helminth, Antigens, Helminth, Enzyme-Linked Immunosorbent Assay, Fasciola hepatica, Fascioliasis, Humans, Parasitology, Sensitivity and Specificity, Serologic Tests

Coprological examination based on egg detection in stool samples is currently used as the gold standard for the diagnosis of human fascioliasis. However, this method is not effective during the acute phase of the disease and has poor sensitivity during the chronic phase. Serodiagnosis has become an excellent alternative to coprological examination in efforts to combat the effects of fascioliasis on human and animal health. Two novel recombinant Fasciola hepatica proteins, i.e., a ferritin (FhFtn-1) and a tegument-associated protein (FhTP16.5), were used as antigens to develop in-house enzyme-linked immunosorbent assay (ELISA) methods. The assays were optimized and validated using 152 serum samples from humans with a known infection status, including healthy subjects, patients with chronic fascioliasis, and patients with other parasitic diseases. The FhFtn-1 ELISA was shown to be 96.6% sensitive and 95.7% specific; the respective parameters for the FhTP16.5 ELISA were 91.4% and 92.4%. The performances of the FhFtn-1 and FhTP16.5 ELISAs were compared with that of an available commercial test (the DRG test) using a subset of serum samples. Our in-house tests were slightly more sensitive than the DRG test in detecting antibodies against F. hepatica, but the differences were not statistically significant. In conclusion, the present study provides evidence for the potential of the FhFtn-1 and FhTP16.5 ELISAs as diagnostic tools for human fascioliasis, as might be implemented in conjunction with standard assays for large-scale screenings in areas where the disease is endemic and for the detection of occasional cases in clinical laboratories.

Alternate JournalJ. Clin. Microbiol.
PubMed ID24353000
PubMed Central IDPMC3957751
Grant List1SC1AI096108-01A2 / AI / NIAID NIH HHS / United States
R25 GM061838 / GM / NIGMS NIH HHS / United States
R25GM061838-13 / GM / NIGMS NIH HHS / United States