Differential effects of dimethyl sulfoxide on nicotinic acetylcholine receptors from mouse muscle and Torpedo electrocytes.

Submitted by Oné R Pagán on

Oné R Pagán's picture
PDF versionPDF version
TitleDifferential effects of dimethyl sulfoxide on nicotinic acetylcholine receptors from mouse muscle and Torpedo electrocytes.
Publication TypeJournal Article
Year of Publication1997
AuthorsEaton, MJ, Pagán, OR, Hann, RM, Eterović, VA
JournalNeurosci Lett
Volume230
Issue3
Pagination163-6
Date Published1997 Jul 25
ISSN0304-3940
KeywordsAdministration, Topical, Animals, Anti-Inflammatory Agents, Bungarotoxins, Dimethyl Sulfoxide, Excitatory Amino Acid Antagonists, Female, Iodine Radioisotopes, Ion Channel Gating, Mice, Oocytes, Patch-Clamp Techniques, Phencyclidine, Radioligand Assay, Receptors, Nicotinic, Torpedo, Tritium, Xenopus laevis
Abstract

The present study examined the effects of 0.1% dimethyl sulfoxide (DMSO) on nicotinic acetylcholine receptors (nAChR) from mouse muscle and Torpedo californica electrocytes. Receptors were expressed in Xenopus laevis oocytes and studied with voltage-clamp. When applied simultaneously with acetylcholine, DMSO did not inhibit current amplitude of either receptor. Preincubation with DMSO for 1 min reduced current amplitude by approximately 50% from oocytes expressing electrocyte receptor. Preincubation did not affect the muscle receptor. With electric organ membranes, 0.1% DMSO did not block either [alpha-(125)I]bungarotoxin binding to the nAChR agonist site or [3H]phencyclidine binding to its high affinity site on resting or desensitized receptor. These data suggest that DMSO might be affecting the electrocyte receptor through a second messenger system.
Alternate JournalNeurosci. Lett.
PubMed ID9272686
Grant ListG12RR03035 / RR / NCRR NIH HHS / United States
R01GM52277 / GM / NIGMS NIH HHS / United States