A functional genetic approach suggests a novel interaction between the human immunodeficiency virus type 1 (HIV-1) Tat protein and HIV-1 TAR RNA in vivo.
Submitted by Mariano Garcia-Blanco on
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Title | A functional genetic approach suggests a novel interaction between the human immunodeficiency virus type 1 (HIV-1) Tat protein and HIV-1 TAR RNA in vivo. |
Publication Type | Journal Article |
Year of Publication | 2003 |
Authors | Lund, LH, Wahren, B, García-Blanco, MA |
Journal | J Gen Virol |
Volume | 84 |
Issue | Pt 3 |
Pagination | 603-6 |
Date Published | 2003 Mar |
ISSN | 0022-1317 |
Keywords | Animals, Cell Line, Cyclin T, Cyclins, Dogs, Gene Products, tat, HIV Long Terminal Repeat, HIV-1, Infectious Anemia Virus, Equine, Models, Molecular, Protein Binding, Recombination, Genetic, RNA, Viral, tat Gene Products, Human Immunodeficiency Virus, Transcriptional Activation |
Abstract | Human immunodeficiency virus type 1 (HIV-1) Tat and human Cyclin T1 form a complex and together recognize the viral TAR RNA element with specificity. Using HIV-1/equine infectious anaemia virus TAR chimeras, we show that in addition to the well-characterized interaction with the bulge, Tat recognizes the distal stem and the loop of TAR. These data support previously proposed, but unproven, molecular models. |
Alternate Journal | J. Gen. Virol. |
PubMed ID | 12604811 |