A New Standard-Based Polynomial Interpolation (SBPIn) method to address gel-to-gel variability for the comparison of multiple denaturing gradient gel electrophoresis profile matrices.

Submitted by Alexis Valentín-Vargas, Ph.D. on

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TitleA New Standard-Based Polynomial Interpolation (SBPIn) method to address gel-to-gel variability for the comparison of multiple denaturing gradient gel electrophoresis profile matrices.
Publication TypeJournal Article
Year of Publication2013
AuthorsValentín-Vargas, A, Chorover, J, Maier, RM
JournalJ Microbiol Methods
Volume92
Issue2
Pagination173-177
Date Published2012 Dec 9
ISSN1872-8359
Abstract

The Standard-Based Polynomial Interpolation (SBPIn) method is a new simple three-step protocol proposed to address common gel-to-gel variations for the comparison of sample profiles across multiple DGGE gels. The advantages of this method include no requirement for additional software or modification of the standard DGGE protocol.
DOI10.1016/j.mimet.2012.12.001
Alternate JournalJ. Microbiol. Methods
PubMed ID23234884