A prospective study of G-CSF primed bone marrow as a stem-cell source for allogeneic bone marrow transplantation in children: a Pediatric Blood and Marrow Transplant Consortium (PBMTC) study.
Submitted by Eneida R Nemecek on
Title | A prospective study of G-CSF primed bone marrow as a stem-cell source for allogeneic bone marrow transplantation in children: a Pediatric Blood and Marrow Transplant Consortium (PBMTC) study. |
Publication Type | Journal Article |
Year of Publication | 2007 |
Authors | Frangoul, H, Nemecek, ER, Billheimer, D, Pulsipher, MA, Khan, S, Woolfrey, A, Manes, B, Cole, C, Walters, MC, Ayas, M, Ravindranath, Y, Levine, JE, Grupp, SA |
Journal | Blood |
Volume | 110 |
Issue | 13 |
Pagination | 4584-7 |
Date Published | 2007 Dec 15 |
ISSN | 0006-4971 |
Keywords | Adolescent, Adult, Antigens, CD34, Bone Marrow, Bone Marrow Transplantation, Cell Count, Child, Child, Preschool, Graft Survival, Graft vs Host Disease, Granulocyte Colony-Stimulating Factor, Hematopoietic Stem Cells, Humans, Infant, Kinetics, Prospective Studies, Survival Analysis, Transplantation, Homologous |
Abstract | A prospective multicenter trial was conducted to evaluate the safety and feasibility of granulocyte colony-stimulating factor (G-CSF)-primed bone marrow (G-BM) in children receiving allogeneic bone marrow transplantation (BMT). A total of 42 children with a median age of 9.8 years (range, 0.8-17 years) were enrolled. Donors with median age of 9.2 years (range, 1.1-22 years) received 5 microg/kg per day of subcutaneous G-CSF for 5 consecutive days. BM was harvested on the fifth day. No donor experienced complications related to G-CSF administration or marrow har-vest. Median nucleated (NC) and CD34 cells infused was 6.7 x 10(8)/kg (range, 2.4-18.5 x 10(8)/kg) and 7.4 x 10(6)/kg (range, 2-27.6 x 10(6)/kg), respectively. Neutrophil and platelet engraftment was at a median of 19 days (range, 13-28 days) and 20 days (range, 9-44 days), respectively. A total of 13 (32%) patients developed grade 2 graft-versus-host disease (GVHD), and 5 (13%) of 40 evaluable patients developed chronic GVHD (3 limited and 2 extensive). Higher cell dose was not associated with increased risk of acute or chronic GVHD. Overall survival and event-free survival at 2 years were 81% and 69%, respectively. Collection of G-BM from pediatric donors is safe, and can result in high NC and CD34 cell doses that facilitate engraftment after myeloablative BMT without a discernable increase in the risk of GVHD. |
DOI | 10.1182/blood-2007-07-101071 |
Alternate Journal | Blood |
PubMed ID | 17827386 |