Standardization of a novel blood-sampling method through the jugular vein for use in the quantified [14C] 2-deoxyglucose method.

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TitleStandardization of a novel blood-sampling method through the jugular vein for use in the quantified [14C] 2-deoxyglucose method.
Publication TypeJournal Article
Year of Publication2006
AuthorsTorres-Reveron, A, Melnick, SM, Stephenson, SI, Dow-Edwards, DL
JournalJ Neurosci Methods
Volume150
Issue2
Pagination143-9
Date Published2006 Jan 30
ISSN0165-0270
KeywordsAnimals, Brain, Carbon Isotopes, Catheters, Indwelling, Deoxyglucose, Femoral Artery, Hematologic Tests, Jugular Veins, Male, Motor Activity, Rats, Rats, Sprague-Dawley
Abstract

In the traditional [14C] deoxyglucose (2DG) method for the measurement of local cerebral glucose utilization (LCGU), blood samples are collected from the femoral artery. However, the placement of a femoral catheter can affect locomotor activity of the animal. We wanted to develop a new technique for blood sampling that would not interfere with the ongoing behavior. Therefore, the present report establishes a method of collecting blood samples for the 2DG method through the jugular vein. To calibrate this method, catheters were inserted in both the femoral artery and jugular vein of adult male Sprague Dawley rats. The next day, rats were injected with 2DG (125 microCi/kg) through the jugular vein. To quantify 14C in plasma, the standard method of blood collection was used for the femoral artery while syringes were used to extract blood samples from the jugular vein. We calculated the integrated specific activity of the plasma and final tissue 2DG concentrations based on Sokoloff's original equation using blood samples derived from both vessels. LCGU determined in selected brain regions was equivalent using both sampling methods. In conclusion, sampling from the jugular vein is appropriate for the quantified 2DG method and does not disrupt locomotor activity of the rat.

DOI10.1016/j.jneumeth.2005.05.018
Alternate JournalJ. Neurosci. Methods
PubMed ID16430967