Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells
Submitted by Beatriz Quiñones on
PDF version| Title | Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells |
| Publication Type | Journal Article |
| Year of Publication | 2003 |
| Authors | Low, SH, Li, X, Miura, M, Kudo, N, Quiñones, B, Weimbs, T |
| Journal | Dev CellDev CellDev Cell |
| Volume | 4 |
| Pagination | 753-9 |
| Date Published | May |
| ISBN Number | 1534-5807 (Print)1534-5807 (Linking) |
| Accession Number | 12737809 |
| Abstract | The terminal step of cytokinesis in animal cells is the abscission of the midbody, a cytoplasmic bridge that connects the two prospective daughter cells. Here we show that two members of the SNARE membrane fusion machinery, syntaxin 2 and endobrevin/VAMP-8, specifically localize to the midbody during cytokinesis in mammalian cells. Inhibition of their function by overexpression of nonmembrane-anchored mutants causes failure of cytokinesis leading to the formation of binucleated cells. Time-lapse microscopy shows that only midbody abscission but not further upstream events, such as furrowing, are affected. These results indicate that successful completion of cytokinesis requires a SNARE-mediated membrane fusion event and that this requirement is distinct from exocytic events that may be involved in prior ingression of the plasma membrane. |
| Short Title | Developmental cellDevelopmental cell |
| Alternate Journal | Developmental cell |
