5' exon replacement and repair by spliceosome-mediated RNA trans-splicing.

Imagen de Mariano Garcia-Blanco
Título5' exon replacement and repair by spliceosome-mediated RNA trans-splicing.
Publication TypeJournal Article
Year of Publication2003
AutoresMansfield, GS, Clark, RH, Puttaraju, M, Kole, J, Cohn, JA, Mitchell, LG, García-Blanco, MA
JournalRNA
Volume9
Issue10
Pagination1290-7
Date Published2003 Oct
ISSN1355-8382
Palabras clavebeta-Galactosidase, Cell Line, Cystic Fibrosis, Cystic Fibrosis Transmembrane Conductance Regulator, DNA Primers, Exons, Humans, Iodides, Ion Channels, Reverse Transcriptase Polymerase Chain Reaction, RNA Precursors, RNA Splice Sites, Spliceosomes, Trans-Splicing, Transfection
Abstract

Spliceosome-mediated RNA trans-splicing (SMaRT) has been used previously to reprogram mutant endogenous CFTR and factor VIII mRNAs in human epithelial cell and tissue models and knockout mice, respectively. Those studies used 3' exon replacement (3'ER); a process in which the distal portion of RNA is reprogrammed. Here, we also show that the 5' end of mRNA can be completely rewritten by 5'ER. For proof-of-concept, and to test whether 5'ER could generate functional CFTR, we generated a mutant minigene target containing CFTR exons 10-24 (deltaF508) and a mini-intron 10, and a pretrans-splicing molecule (targeted to intron 10) containing CFTR exons 1-10 (+F508), and tested these two constructs in 293T cells for anion efflux transport. Cells cotransfected with target and PTM showed a consistent increase in anion efflux, but there was no response in control cells that received PTM or target alone. Using a LacZ reporter system to accurately quantify trans-splicing efficiency, we tested several unique PTM designs. These studies provided two important findings as follows: (1) efficient trans-splicing can be achieved by binding the PTM to different locations in the target, and (2) relatively few changes in PTM design can have a profound impact on trans-splicing activity. Tethering the PTM close to the target 3' splice site (as opposed to the donor site) and inserting an intron in the PTM coding resulted in a 65-fold enhancement of LacZ activity. These studies demonstrate that (1) SMaRT can be used to reprogram the 5' end of mRNA, and (2) efficiency can be improved substantially.

Alternate JournalRNA
PubMed ID13130143