Advanced optical imaging techniques for neurodevelopment.
Enviado por Daniel Alfonso Colón-Ramos el
Título | Advanced optical imaging techniques for neurodevelopment. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Autores | Wu, Y, Christensen, R, Colón-Ramos, DA, Shroff, H |
Journal | Curr Opin Neurobiol |
Volume | 23 |
Issue | 6 |
Pagination | 1090-7 |
Date Published | 2013 Dec |
ISSN | 1873-6882 |
Palabras clave | Animals, Humans, Imaging, Three-Dimensional, Nervous System, Neurogenesis, Optical Imaging |
Abstract | Over the past decade, developmental neuroscience has been transformed by the widespread application of confocal and two-photon fluorescence microscopy. Even greater progress is imminent, as recent innovations in microscopy now enable imaging with increased depth, speed, and spatial resolution; reduced phototoxicity; and in some cases without external fluorescent probes. We discuss these new techniques and emphasize their dramatic impact on neurobiology, including the ability to image neurons at depths exceeding 1mm, to observe neurodevelopment noninvasively throughout embryogenesis, and to visualize neuronal processes or structures that were previously too small or too difficult to target with conventional microscopy. |
DOI | 10.1016/j.conb.2013.06.008 |
Alternate Journal | Curr. Opin. Neurobiol. |
PubMed ID | 23831260 |
PubMed Central ID | PMC3830703 |
Grant List | R01 NS076558 / NS / NINDS NIH HHS / United States ZIA EB000074-01 / / Intramural NIH HHS / United States |