Advanced optical imaging techniques for neurodevelopment.

Imagen de Daniel Alfonso Colón-Ramos
PDF versionPDF version
TítuloAdvanced optical imaging techniques for neurodevelopment.
Publication TypeJournal Article
Year of Publication2013
AutoresWu, Y, Christensen, R, Colón-Ramos, DA, Shroff, H
JournalCurr Opin Neurobiol
Date Published2013 Dec
Palabras claveAnimals, Humans, Imaging, Three-Dimensional, Nervous System, Neurogenesis, Optical Imaging

Over the past decade, developmental neuroscience has been transformed by the widespread application of confocal and two-photon fluorescence microscopy. Even greater progress is imminent, as recent innovations in microscopy now enable imaging with increased depth, speed, and spatial resolution; reduced phototoxicity; and in some cases without external fluorescent probes. We discuss these new techniques and emphasize their dramatic impact on neurobiology, including the ability to image neurons at depths exceeding 1mm, to observe neurodevelopment noninvasively throughout embryogenesis, and to visualize neuronal processes or structures that were previously too small or too difficult to target with conventional microscopy.

Alternate JournalCurr. Opin. Neurobiol.
PubMed ID23831260
PubMed Central IDPMC3830703
Grant ListR01 NS076558 / NS / NINDS NIH HHS / United States
ZIA EB000074-01 / / Intramural NIH HHS / United States