Altered subcellular signaling in murine peritoneal macrophages upon chronic morphine exposure.
Enviado por Cristhian J Ildefonso el
Título | Altered subcellular signaling in murine peritoneal macrophages upon chronic morphine exposure. |
Publication Type | Journal Article |
Year of Publication | 2006 |
Autores | Lugo-Chinchilla, AM, Báez, D, Vélez, M, Ildefonso, C, Renaud, FL |
Journal | J Neuroimmunol |
Volume | 176 |
Issue | 1-2 |
Pagination | 86-94 |
Date Published | 2006 Jul |
ISSN | 0165-5728 |
Palabras clave | Adenylate Cyclase, Animals, Cyclic AMP, Cyclic AMP-Dependent Protein Kinases, Female, Macrophages, Peritoneal, Mice, Mice, Inbred C3H, Morphine, Pertussis Toxin, Phagocytosis, Signal Transduction |
Abstract | Alterations in opioid signaling that take place in murine peritoneal macrophages in vitro are variably dependent on opiate exposure conditions. Acute exposure to morphine inhibits Fc-mediated phagocytosis by a pertussis toxin (PT)-sensitive mechanism, but has no effect on cAMP levels. In contrast, chronic exposure to morphine results in a "tolerant" state, wherein test and control values for both phagocytosis and cAMP are equivalent. However, drug withdrawal after chronic exposure to morphine results in inhibition of phagocytosis and a concomitant 4-fold increase in cAMP by a PT-insensitive mechanism. This increase is causally related to inhibition of phagocytosis since an artificial increase in cAMP inhibits phagocytosis in non-withdrawn cells exposed chronically to morphine. We suggest that macrophage opioid receptors signaling switches from a Gi/o-mediated mechanism that does not involve adenylate cyclase in acute exposure to a non-Gi/o-mediated adenylate cyclase superactivation during chronic exposure. |
DOI | 10.1016/j.jneuroim.2006.04.007 |
Alternate Journal | J. Neuroimmunol. |
PubMed ID | 16750574 |
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