Antibiotic-resistance and virulence genes in Enterococcus isolated from tropical recreational waters.
Enviado por Tasha Marie Santiago Rodriguez el
Título | Antibiotic-resistance and virulence genes in Enterococcus isolated from tropical recreational waters. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Autores | Santiago-Rodriguez, TM, Rivera, JI, Coradin, M, Toranzos, GA |
Journal | J Water Health |
Volume | 11 |
Issue | 3 |
Pagination | 387-96 |
Date Published | 2013 Sep |
ISSN | 1477-8920 |
Palabras clave | DNA, Bacterial, Enterococcus faecalis, Polymerase Chain Reaction, Puerto Rico, Recreation, Silicon Dioxide, Tetracycline Resistance, Tropical Climate, Vancomycin Resistance, Virulence, Water Microbiology |
Abstract | The prevalence of enterococci harboring tetracycline- and vancomycin-resistance genes, as well as the enterococcal surface protein (esp) has mostly been determined in clinical settings, but their prevalence in tropical recreational waters remains largely unknown. The present study determined the prevalence of tetM (tetracycline-resistance), vanA and vanB (vancomycin-resistance) in the bacterial and viral fractions, enterococci and their induced phages isolated from tropical recreational marine and fresh waters, dry and wet sands. Since lysogenic phages can act as vectors for antibiotic-resistance and virulence factors, the prevalence of the mentioned genes, as well as that of an integrase-encoding gene (int) specific for Enterococcus faecalis phages was determined. Up to 60 and 54% of the bacterial fractions and enterococci, respectively, harbored at least one of the tested genes suggesting that bacteria in tropical environments may be reservoirs of antibiotic-resistance and virulence genes. int was detected in the viral fractions and in one Enterococcus isolate after induction. This study presents the opportunity to determine if the presence of bacteria harboring antibiotic-resistance and virulence genes in tropical recreational waters represents a threat to public health. |
DOI | 10.2166/wh.2013.005 |
Alternate Journal | J Water Health |
PubMed ID | 23981868 |
PubMed Central ID | PMC4096248 |
Grant List | 2R25GM061151-09 / GM / NIGMS NIH HHS / United States P20 RR16470 / RR / NCRR NIH HHS / United States R25 GM061151 / GM / NIGMS NIH HHS / United States S06GM08102 / GM / NIGMS NIH HHS / United States T34 GM007821 / GM / NIGMS NIH HHS / United States |