The anticancer activity of chloroquine-gold nanoparticles against MCF-7 breast cancer cells
Enviado por Jaime E Ramirez-Vick el
PDF version| Título | The anticancer activity of chloroquine-gold nanoparticles against MCF-7 breast cancer cells |
| Publication Type | Journal Article |
| Year of Publication | 2012 |
| Autores | Joshi, P, Chakraborti, S, Ramirez-Vick, JE, Ansari, ZA, Shanker, V, Chakrabarti, P, Singh, SP |
| Journal | Colloids Surf B BiointerfacesColloids Surf B BiointerfacesColloids Surf B Biointerfaces |
| Volume | 95 |
| Pagination | 195-200 |
| Date Published | Jun 15 |
| Type of Article | Research Support, Non-U.S. Gov't |
| ISBN Number | 1873-4367 (Electronic)<br/>0927-7765 (Linking) |
| Accession Number | 22445746 |
| Abstract | In the present study, 11-mercaptoundecanoic acid-modified gold nanoparticles ( approximately 7 nm) were conjugated with chloroquine to explore their potential application in cancer therapeutics. The anticancer activity of chloroquine-gold nanoparticle conjugates (GNP-Chl) was demonstrated in MCF-7 breast cancer cells. The MCF-7 cells were treated with different concentrations of GNP-Chl conjugates, and the cell viability was assayed using trypan blue, resulting in an IC(50) value of 30 +/- 5 mug/mL. Flow cytometry analysis revealed that the major pathway of cell death was necrosis, which was mediated by autophagy. The drug release kinetics of GNP-Chl conjugates revealed the release of chloroquine at an acidic pH, which was quantitatively estimated using optical absorbance spectroscopy. The nature of stimuli-responsive drug release and the inhibition of cancer cell growth by GNP-Chl conjugates could pave the way for the design of combinatorial therapeutic agents, particularly nanomedicine, for the treatment of cancer. |
| Short Title | Colloids and surfaces. B, BiointerfacesColloids and surfaces. B, Biointerfaces |
| Alternate Journal | Colloids and surfaces. B, Biointerfaces |
