A cell-permeable peptide inhibits hepatitis C virus replication by sequestering IRES transacting factors.
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Título | A cell-permeable peptide inhibits hepatitis C virus replication by sequestering IRES transacting factors. |
Publication Type | Journal Article |
Year of Publication | 2009 |
Autores | Fontanes, V, Raychaudhuri, S, Dasgupta, A |
Journal | Virology |
Volume | 394 |
Issue | 1 |
Pagination | 82-90 |
Date Published | 2009 Nov 10 |
ISSN | 1096-0341 |
Abstract | Hepatitis C virus (HCV) infection frequently leads to chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. There is no effective therapy or vaccine available to HCV-infected patients other than interferon-ribavarin combination, which is effective in a relatively small percentage of infected patients. Our previous results have shown that a synthetic peptide (LAP) corresponding to the N-terminal 18 amino acids of the Lupus autoantigen (La) was a potent inhibitor of HCV IRES-mediated translation. We demonstrate here that LAP efficiently blocks HCV replication of infectious JFH1 virus in cell culture. Our data suggest that LAP forms complexes with IRES-transacting factors (ITAFs) PTB and PCBP2. LAP-mediated inhibition of HCV IRES-mediated translation in vitro could be fully rescued by recombinant PCB and PCBP2. Also transient expression of PTB / PCBP2 combination significantly restores HCV replication in LAP-inhibited cultures. These results suggest that ITAFs could be potential targets to block HCV replication. |
DOI | 10.1016/j.virol.2009.08.012 |
Alternate Journal | Virology |
PubMed ID | 19740508 |
PubMed Central ID | PMC2767405 |
Grant List | AI-38056 / AI / NIAID NIH HHS / United States AI-45188 / AI / NIAID NIH HHS / United States AI-45733 / AI / NIAID NIH HHS / United States R01 AI045733 / AI / NIAID NIH HHS / United States R01 AI045733-05 / AI / NIAID NIH HHS / United States R41 AI045188 / AI / NIAID NIH HHS / United States R41 AI045188-01 / AI / NIAID NIH HHS / United States |