Cerebrospinal fluid proteomic profiling of HIV-1-infected patients with cognitive impairment.
Enviado por Loyda Milagros Melendez, Ph.D. el
Título | Cerebrospinal fluid proteomic profiling of HIV-1-infected patients with cognitive impairment. |
Publication Type | Journal Article |
Year of Publication | 2007 |
Autores | Rozek, W, Ricardo-Dukelow, M, Holloway, S, Gendelman, HE, Wojna, V, Melendez, LM, Ciborowski, P |
Journal | J Proteome Res |
Volume | 6 |
Issue | 11 |
Pagination | 4189-99 |
Date Published | 2007 Nov |
ISSN | 1535-3893 |
Palabras clave | Amino Acid Sequence, Blotting, Western, Cerebrospinal Fluid, Cognition Disorders, Complement C3, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Fluorescent Dyes, Gene Expression Profiling, HIV Infections, HIV-1, Humans, Mass Spectrometry, Molecular Sequence Data, Proteomics |
Abstract | Advanced HIV-1 infection is commonly associated with progressive immune suppression and the development of cognitive, motor, and behavior disturbances. In its most severe form, it is diagnosed as HIV-1 associated dementia (HAD) and can progress to profound functional disability and death. Despite prodigious efforts to uncover biomarkers of HAD, none can adequately reflect disease onset or progression. Thus, we developed a proteomics platform for HAD biomarker discovery and used it to perform a pilot study on cerebrospinal fluid (CSF) from HIV-1-infected people with or without HAD. A 2-dimensional electrophoresis (2-DE) map of a HAD CSF proteome was focused on differentially expressed proteins. 2-DE difference gel electrophoresis (2-D DIGE) analysis showed >90 differences in protein spots of which 20 proteins were identified. Differential expression of 6 proteins was validated by Western blot tests and included vitamin D binding protein, clusterin, gelsolin, complement C3, procollagen C-endopeptidase enhancer 1, and cystatin C. We posit that these proteins, alone or together, are potential HAD biomarkers. |
DOI | 10.1021/pr070220c |
Alternate Journal | J. Proteome Res. |
PubMed ID | 17929958 |
Grant List | 1P20RR11126 / RR / NCRR NIH HHS / United States 1R21 MH075662-01 / MH / NIMH NIH HHS / United States 1T32 NS07488 / NS / NINDS NIH HHS / United States 20 RR15635 / RR / NCRR NIH HHS / United States P20 RR011126 / RR / NCRR NIH HHS / United States R21 MH075662 / MH / NIMH NIH HHS / United States S11 NS046278 / NS / NINDS NIH HHS / United States U54 NS043011 / NS / NINDS NIH HHS / United States U54 NS4301 / NS / NINDS NIH HHS / United States |