Characterization of the placental macrophage secretome: implications for antiviral activity.

Imagen de Loyda Milagros Melendez, Ph.D.
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TítuloCharacterization of the placental macrophage secretome: implications for antiviral activity.
Publication TypeJournal Article
Year of Publication2009
AutoresGarcía, K, García, V, J Laspiur, P, Duan, F, Meléndez, LM
JournalPlacenta
Volume30
Issue2
Pagination149-55
Date Published2009 Feb
ISSN0143-4004
Palabras claveAdult, Blotting, Western, Cells, Cultured, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, HIV Infections, Humans, Macrophages, Peroxiredoxins, Placenta, Pregnancy, Protein Array Analysis, Proteins, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry, Virus Replication, Young Adult
Abstract

It is well documented that placental macrophages show lower levels of HIV-1 infection than monocyte-derived macrophages (MDM). We used proteomic methods to test the hypothesis that placental macrophages secrete different proteins as compared to MDM that may contribute to decreased HIV-1 replication. Placental macrophages and MDM were cultured for 12 days and supernatant was collected. To characterize supernatants, the protein profiles of placental macrophages and MDM were compared using the protein chip assay. Subsequently, proteins were separated by one-dimensional gel electrophoresis and identified by tandem mass spectrometry at the corresponding mass to charge (m/z) range of 5000-20,000. Significant differences were found between placental macrophages and MDM in seven protein peaks with m/z values of 6075, 6227, 11,662, 14,547, 6158, 7740, and 11,934 on the CM10 and IMAC chips. After sequencing and identification, five proteins were validated for differential expression in placental macrophages and MDM by Western blot analyses. Peroxiredoxin 5, found to be more abundant in placental macrophage supernatants, is important in the cellular antioxidant mechanisms, and other members of its family have shown antiviral activity. Cystatin B was less abundant in PM supernatant, and decreased intracellular levels have recently been shown to be associated with lower HIV-1 replication in placental macrophages than in MDM. This study elucidates for the first time the placental macrophage secretome corresponding to 5000-20,000 Da and advances our understanding of the proteins secreted in the placenta that can protect the fetus against HIV-1 and other viral infections.

DOI10.1016/j.placenta.2008.10.014
Alternate JournalPlacenta
PubMed ID19070362
PubMed Central IDPMC2947718
Grant ListG12 RR003051 / RR / NCRR NIH HHS / United States
G12 RR003051-235483 / RR / NCRR NIH HHS / United States
G12RR-03051 / RR / NCRR NIH HHS / United States
GM61838 / GM / NIGMS NIH HHS / United States
R25 GM061838 / GM / NIGMS NIH HHS / United States
R25 GM061838-09 / GM / NIGMS NIH HHS / United States
S06 GM008224 / GM / NIGMS NIH HHS / United States
S06 GM008224-19 / GM / NIGMS NIH HHS / United States
S06GM0822 / GM / NIGMS NIH HHS / United States
U54 NS043011 / NS / NINDS NIH HHS / United States
U54 NS043011-01 / NS / NINDS NIH HHS / United States
U54NS430 / NS / NINDS NIH HHS / United States