A functional genetic approach suggests a novel interaction between the human immunodeficiency virus type 1 (HIV-1) Tat protein and HIV-1 TAR RNA in vivo.

Imagen de Mariano Garcia-Blanco
TítuloA functional genetic approach suggests a novel interaction between the human immunodeficiency virus type 1 (HIV-1) Tat protein and HIV-1 TAR RNA in vivo.
Publication TypeJournal Article
Year of Publication2003
AutoresLund, LH, Wahren, B, García-Blanco, MA
JournalJ Gen Virol
Volume84
IssuePt 3
Pagination603-6
Date Published2003 Mar
ISSN0022-1317
Palabras claveAnimals, Cell Line, Cyclin T, Cyclins, Dogs, Gene Products, tat, HIV Long Terminal Repeat, HIV-1, Infectious Anemia Virus, Equine, Models, Molecular, Protein Binding, Recombination, Genetic, RNA, Viral, tat Gene Products, Human Immunodeficiency Virus, Transcriptional Activation
Abstract

Human immunodeficiency virus type 1 (HIV-1) Tat and human Cyclin T1 form a complex and together recognize the viral TAR RNA element with specificity. Using HIV-1/equine infectious anaemia virus TAR chimeras, we show that in addition to the well-characterized interaction with the bulge, Tat recognizes the distal stem and the loop of TAR. These data support previously proposed, but unproven, molecular models.

Alternate JournalJ. Gen. Virol.
PubMed ID12604811