Imaging alternative splicing in living cells.

Imagen de Mariano Garcia-Blanco
TítuloImaging alternative splicing in living cells.
Publication TypeJournal Article
Year of Publication2004
AutoresWagner, EJ, Baines, A, Albrecht, T, Brazas, R, García-Blanco, MA
JournalMethods Mol Biol
Volume257
Pagination29-46
Date Published2004
ISSN1064-3745
Palabras claveAlternative Splicing, Animals, Cells, Cultured, Exons, Green Fluorescent Proteins, Humans, Introns, Kidney, Luminescent Proteins, Male, Microscopy, Fluorescence, Prostatic Neoplasms, Rats, Receptor Protein-Tyrosine Kinases, Receptor, Fibroblast Growth Factor, Type 2, Receptors, Fibroblast Growth Factor, RNA Precursors
Abstract

We have developed an in vivo reporter of alternative splicing decisions that allows for the determination of FGF-R2 splicing patterns without the destruction of cells. This method has broad applications, including the study of other alternatively spliced genes in tissue culture and in whole animals, and may be useful in creating imaging markers for the study of tumor progression and metastasis. In this chapter, the authors present one example of this method using fluorescence reporters. As with any new assay, a series of experiments were performed to validate the method. This chapter documents some of these experiments.

DOI10.1385/1-59259-750-5:029
Alternate JournalMethods Mol. Biol.
PubMed ID14769994