Immunoprecipitation of bovine brain membranes enriched in M1 and M2 muscarinic receptors with monoclonal antibody 10C7.
Enviado por Walter Silva el
Título | Immunoprecipitation of bovine brain membranes enriched in M1 and M2 muscarinic receptors with monoclonal antibody 10C7. |
Publication Type | Journal Article |
Year of Publication | 1990 |
Autores | Silva, WI, Kohtz, DS, Puszkin, S |
Journal | Neurosci Lett |
Volume | 113 |
Issue | 1 |
Pagination | 89-94 |
Date Published | 1990 May 18 |
ISSN | 0304-3940 |
Palabras clave | Animals, Antibodies, Monoclonal, Binding, Competitive, Brain, Cattle, Pirenzepine, Precipitin Tests, Quinuclidinyl Benzilate, Receptors, Muscarinic, Subcellular Fractions |
Abstract | Monoclonal antibodies prepared against clathrin-coated vesicles cargo molecules were screened for their ability to precipitate the mAChR binding activity present in a light-density smooth membrane fraction from bovine brain called peak I. Only monoclonal antibody 10C7 was able to selectively sediment 38% (+/- 4.2) of the mAChR binding activity of peak I. Analysis by competition experiments of the supernatant obtained after the immunoprecipitation step reveals that neither the ratio of high/low affinity sites, nor the affinity ratio KH/KL was significantly altered for either of the muscarinic antagonists. The data implies that the bovine brain smooth-membrane compartment(s) expressing the mAb 10C7 antigen is also enriched in M1 and M2 mAChR. |
Alternate Journal | Neurosci. Lett. |
PubMed ID | 2366954 |
Grant List | 1-G-12-RR03035 / RR / NCRR NIH HHS / United States 1-R29-NS27259 / NS / NINDS NIH HHS / United States HL-40659 / HL / NHLBI NIH HHS / United States |