Macrophage proteomic fingerprinting predicts HIV-1-associated cognitive impairment.

Imagen de Loyda Milagros Melendez, Ph.D.
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TítuloMacrophage proteomic fingerprinting predicts HIV-1-associated cognitive impairment.
Publication TypeJournal Article
Year of Publication2003
AutoresLuo, X, Carlson, KA, Wojna, V, Mayo, R, Biskup, TM, Stoner, J, Anderson, J, Gendelman, HE, Meléndez, LM
JournalNeurology
Volume60
Issue12
Pagination1931-7
Date Published2003 Jun 24
ISSN1526-632X
Palabras claveAdult, AIDS Dementia Complex, CD4 Lymphocyte Count, Cell Differentiation, Cognition, Cohort Studies, Female, HIV-1, Humans, Macrophages, Middle Aged, Monocytes, Neuropsychological Tests, Protein Array Analysis, Proteomics, Puerto Rico, Sensitivity and Specificity, Subtraction Technique, Viral Load
Abstract

BACKGROUND: Specific proteins produced from monocytes may be linked to the pathogenesis and aid in the diagnosis of HIV-1-associated dementia (HAD).

OBJECTIVE: The authors assessed whether a diagnostic phenomic protein profile could be obtained from monocyte-derived macrophages (MDM) from HIV-1-infected patients with cognitive impairment.

METHODS: Twenty-one HIV-1-infected Hispanic women and 10 seronegative controls matched by age and sex were followed at the University of Puerto Rico Medical Sciences Campus, where neuropsychological, immune, and viral parameters were tested. Monocytes were recovered by Percoll gradient centrifugation from peripheral blood mononuclear cells. MDM lysates were prepared after 7 days of cultivation and protein profiles analyzed by surface enhanced laser desorption/ionization (SELDI)-time of flight (TOF) ProteinChip tests. Classification trees were prepared for statistical analyses.

RESULTS: A total of 177 protein peaks from 2 to 80 kDa were evaluated in 31 patient MDM lysates by SELDI-TOF ProteinChip assays. Select protein peaks, at 5028 and 4320 Da, separated HIV-1-infected from HIV-1-seronegative subjects with a sensitivity of 100% and a specificity of 80%. Thirty-eight peaks were used to differentiate HIV-1-infected subjects with and without cognitive impairment. A 4348 Da protein separated the two groups with a sensitivity of 100% and a specificity of 75%.

CONCLUSIONS: The identification of unique phenomic MDM profiles from cognitively impaired HIV-1-infected patients supports the hypothesis that changes in monocyte function parallel the development of HAD.

Alternate JournalNeurology
PubMed ID12821735
Grant List1 U54 NS43011 / NS / NINDS NIH HHS / United States
2R01 NS34239 / NS / NINDS NIH HHS / United States
2R37 NS36136 / NS / NINDS NIH HHS / United States
G12 RR-03051 / RR / NCRR NIH HHS / United States
P01 NS31492 / NS / NINDS NIH HHS / United States