Mouse models of osteoarthritis: surgical model of posttraumatic osteoarthritis induced by destabilization of the medial meniscus.

Imagen de Miguel Otero
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TítuloMouse models of osteoarthritis: surgical model of posttraumatic osteoarthritis induced by destabilization of the medial meniscus.
Publication TypeJournal Article
Year of Publication2015
AutoresCulley, KL, Dragomir, CL, Chang, J, Wondimu, EB, Coico, J, Plumb, DA, Otero, M, Goldring, MB
JournalMethods Mol Biol
Volume1226
Pagination143-73
Date Published2015
ISSN1940-6029
Abstract

The surgical model of destabilization of the medial meniscus (DMM) has become a gold standard for studying the onset and progression of posttraumatic osteoarthritis (OA). The DMM model mimics clinical meniscal injury, a known predisposing factor for the development of human OA, and permits the study of structural and biological changes over the course of the disease. In addition, when applied to genetically modified or engineered mouse models, this surgical procedure permits dissection of the relative contribution of a given gene to OA initiation and/or progression. This chapter describes the requirements for the surgical induction of OA in mouse models, and provides guidelines and tools for the subsequent histological, immunohistochemical, and molecular analyses. Methods for the assessment of the contributions of selected genes in genetically modified strains are also provided.

DOI10.1007/978-1-4939-1619-1_12
Alternate JournalMethods Mol. Biol.
PubMed ID25331049
Grant ListR01-AG-022021 / AG / NIA NIH HHS / United States
RC4-AR060546 / AR / NIAMS NIH HHS / United States