Netrin (UNC-6) mediates dendritic self-avoidance.
Enviado por Daniel Alfonso Colón-Ramos el
Título | Netrin (UNC-6) mediates dendritic self-avoidance. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Autores | Smith, CJ, Watson, JD, VanHoven, MK, Colón-Ramos, DA, Miller, DM |
Journal | Nat Neurosci |
Volume | 15 |
Issue | 5 |
Pagination | 731-7 |
Date Published | 2012 May |
ISSN | 1546-1726 |
Palabras clave | Animals, Animals, Genetically Modified, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cell Adhesion Molecules, Cell Movement, Cloning, Molecular, Dendrites, Hot Temperature, Luminescent Proteins, Membrane Proteins, Microscopy, Confocal, Models, Molecular, Mutation, Nerve Tissue Proteins, Nociceptors, Signal Transduction, Time Factors, Time-Lapse Imaging |
Abstract | Dendrites from a single neuron may be highly branched but typically do not overlap. Self-avoidance behavior has been shown to depend on cell-specific membrane proteins that trigger mutual repulsion. Here we report the unexpected discovery that a diffusible cue, the axon guidance protein UNC-6 (Netrin), is required for self-avoidance of sister dendrites from the PVD nociceptive neuron in Caenorhabditis elegans. We used time-lapse imaging to show that dendrites fail to withdraw upon mutual contact in the absence of UNC-6 signaling. We propose a model in which the UNC-40 (Deleted in Colorectal Cancer; DCC) receptor captures UNC-6 at the tips of growing dendrites for interaction with UNC-5 on the apposing branch to induce mutual repulsion. UNC-40 also responds to dendritic contact through another pathway that is independent of UNC-6. Our findings offer a new model for how an evolutionarily conserved morphogenic cue and its cognate receptors can pattern a fundamental feature of dendritic architecture. |
DOI | 10.1038/nn.3065 |
Alternate Journal | Nat. Neurosci. |
PubMed ID | 22426253 |