Neural processing of a cocaine-associated odor cue revealed by functional MRI in awake rats
Enviado por Marcelo Febo el
PDF version| Título | Neural processing of a cocaine-associated odor cue revealed by functional MRI in awake rats |
| Publication Type | Journal Article |
| Autores | Johnson, TR, Smerkers, B, Moulder, JK, Stellar, JR, Febo, M |
| Journal | Neurosci LettNeurosci Lett |
| Volume | 534 |
| Pagination | 160-5 |
| Date Published | Feb 8 |
| ISBN Number | 1872-7972 (Electronic)<br/>0304-3940 (Linking) |
| Accession Number | 23262077 |
| Abstract | Using an olfactory conditioning procedure, brain stimulation reward threshold measurements, and functional magnetic resonance imaging (fMRI), we investigated brain stimulation reward threshold change and fMRI neural activation in response to a cocaine-associated odor cue. In the first brain stimulation experiment, over 10 days of rate-frequency curve-shift testing, rats were administered intravenous cocaine (1.0mg/kg) paired with a contextual cue of peppermint odor previously placed in the operant chamber or they were given vehicle treatment (no cocaine) in the presence of no olfactory cue. Following a 14-day drug-free rest period, rats were again given the rate-frequency curve-shift threshold test with or without the odor cue. In a second experiment, rats were similarly conditioned with a peppermint odor but with intraperitoneally delivered cocaine (10mg/kg). After a 14 day rest period, rats were imaged on a 7-T MRI for their blood oxygen level dependent (BOLD) in response to the cocaine-paired peppermint odor versus an unpaired neutral lemon odor. In the brain stimulation experiment, expected significant reward threshold shifts were produced by cocaine and, importantly, about half that level of shift was produced by the paired contextual olfactory cue. In the fMRI experiment, the insular cortex showed a significantly greater BOLD activation in cocaine-treated versus saline-treated animals to the olfactory cue, but not with the unpaired lemon scent. These data are in agreement with previous studies suggesting a role of the insular cortex in attributing reward value (positive or negative) to conditioned odor stimuli. |
