Purification and characterization of liver arylformamidase in rainbow trout and cattle.

Imagen de Adelfa Serrano-Brizuela
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TítuloPurification and characterization of liver arylformamidase in rainbow trout and cattle.
Publication TypeJournal Article
Year of Publication1991
AutoresSerrano, AE, Nagayama, F
JournalComp Biochem Physiol B
Volume98
Issue2-3
Pagination381-7
Date Published1991
ISSN0305-0491
Palabras claveAnimals, Arylformamidase, Cattle, Chromatography, Hydrogen-Ion Concentration, Kinetics, Kynurenine, Liver, Molecular Weight, Species Specificity, Temperature, Trout
Abstract

1. Arylformamidases were purified from the liver of rainbow trout and cattle. 2. Optimal pH's were 7.7 and 8.0 for the fish and cattle enzyme, respectively. Both enzymes showed optimum temperature at 40 degrees C. Thermal and pH stability ranges and Arrhenius plots of the enzymes differed. 3. Km and molecular weight of the fish enzyme were determined to be 0.28 mM and 32,700, respectively, while those for the cattle enzyme were 0.78 mM and 42,5000, respectively.

Alternate JournalComp. Biochem. Physiol., B
PubMed ID1714804