Purification and characterization of liver arylformamidase in rainbow trout and cattle.
Enviado por Adelfa Serrano-Brizuela el
Título | Purification and characterization of liver arylformamidase in rainbow trout and cattle. |
Publication Type | Journal Article |
Year of Publication | 1991 |
Autores | Serrano, AE, Nagayama, F |
Journal | Comp Biochem Physiol B |
Volume | 98 |
Issue | 2-3 |
Pagination | 381-7 |
Date Published | 1991 |
ISSN | 0305-0491 |
Palabras clave | Animals, Arylformamidase, Cattle, Chromatography, Hydrogen-Ion Concentration, Kinetics, Kynurenine, Liver, Molecular Weight, Species Specificity, Temperature, Trout |
Abstract | 1. Arylformamidases were purified from the liver of rainbow trout and cattle. 2. Optimal pH's were 7.7 and 8.0 for the fish and cattle enzyme, respectively. Both enzymes showed optimum temperature at 40 degrees C. Thermal and pH stability ranges and Arrhenius plots of the enzymes differed. 3. Km and molecular weight of the fish enzyme were determined to be 0.28 mM and 32,700, respectively, while those for the cattle enzyme were 0.78 mM and 42,5000, respectively. |
Alternate Journal | Comp. Biochem. Physiol., B |
PubMed ID | 1714804 |