Ralstonia solanacearum encounters an oxidative environment during tomato infection.
Enviado por Zomary Flores-Cruz el
Título | Ralstonia solanacearum encounters an oxidative environment during tomato infection. |
Publication Type | Journal Article |
Year of Publication | 2009 |
Autores | Flores-Cruz, Z, Allen, C |
Journal | Mol Plant Microbe Interact |
Volume | 22 |
Issue | 7 |
Pagination | 773-82 |
Date Published | 2009 Jul |
ISSN | 0894-0282 |
Palabras clave | Gene Expression Regulation, Bacterial, Genes, Bacterial, Genome, Bacterial, Hydrogen Peroxide, Lycopersicon esculentum, Mutation, Oxidative Stress, Plant Diseases, Plant Leaves, Ralstonia solanacearum, Reactive Oxygen Species |
Abstract | Ralstonia solanacearum genes that are induced during tomato infection suggested that this pathogen encounters reactive oxygen species (ROS) during bacterial wilt pathogenesis. The genomes of R. solanacearum contain multiple redundant ROS-scavenging enzymes, indirect evidence that this pathogen experiences intense oxidative stress during its life cycle. Over 9% of the bacterium's plant-induced genes were also upregulated by hydrogen peroxide in culture, suggesting that oxidative stress may be linked to life in the plant host. Tomato leaves infected by R. solanacearum contained hydrogen peroxide, and concentrations of this ROS increased as pathogen populations increased. Mutagenesis of a plant-induced predicted peroxidase gene, bcp, resulted in an R. solanacearum strain with reduced ability to detoxify ROS in culture. The bcp mutant caused slightly delayed bacterial wilt disease onset in tomato. Moreover, its virulence was significantly reduced on tobacco plants engineered to overproduce hydrogen peroxide, demonstrating that Bcp is necessary for detoxification of plant-derived hydrogen peroxide and providing evidence that host ROS can limit the success of this pathogen. These results reveal that R. solanacearum is exposed to ROS during pathogenesis and that it has evolved a redundant and efficient oxidative stress response to adapt to the host environment and cause disease. |
DOI | 10.1094/MPMI-22-7-0773 |
Alternate Journal | Mol. Plant Microbe Interact. |
PubMed ID | 19522559 |