Reconstruction of the chemotaxis receptor-kinase assembly.
Enviado por Gabriela Gonzalez Bonet el
Título | Reconstruction of the chemotaxis receptor-kinase assembly. |
Publication Type | Journal Article |
Year of Publication | 2006 |
Autores | Park, S-Y, Borbat, PP, Gonzalez-Bonet, G, Bhatnagar, J, Pollard, AM, Freed, JH, Bilwes, AM, Crane, BR |
Journal | Nat Struct Mol Biol |
Volume | 13 |
Issue | 5 |
Pagination | 400-7 |
Date Published | 2006 May |
ISSN | 1545-9993 |
Palabras clave | Bacterial Proteins, Chemotaxis, Crystallography, X-Ray, Cytoplasm, Dimerization, Electron Spin Resonance Spectroscopy, Membrane Proteins, Models, Molecular, Protein Binding, Protein Folding, Protein Kinases, Protein Structure, Quaternary, Signal Transduction, Thermotoga maritima |
Abstract | In bacterial chemotaxis, an assembly of transmembrane receptors, the CheA histidine kinase and the adaptor protein CheW processes environmental stimuli to regulate motility. The structure of a Thermotoga maritima receptor cytoplasmic domain defines CheA interaction regions and metal ion-coordinating charge centers that undergo chemical modification to tune receptor response. Dimeric CheA-CheW, defined by crystallography and pulsed ESR, positions two CheWs to form a cleft that is lined with residues important for receptor interactions and sized to clamp one receptor dimer. CheW residues involved in kinase activation map to interfaces that orient the CheW clamps. CheA regulatory domains associate in crystals through conserved hydrophobic surfaces. Such CheA self-contacts align the CheW receptor clamps for binding receptor tips. Linking layers of ternary complexes with close-packed receptors generates a lattice with reasonable component ratios, cooperative interactions among receptors and accessible sites for modification enzymes. |
DOI | 10.1038/nsmb1085 |
Alternate Journal | Nat. Struct. Mol. Biol. |
PubMed ID | 16622408 |
Grant List | GM:R01066775 / GM / NIGMS NIH HHS / United States P41 RR016292 / RR / NCRR NIH HHS / United States P41 RR016292-05 / RR / NCRR NIH HHS / United States P41 RR016292-06 / RR / NCRR NIH HHS / United States P41-RR016292 / RR / NCRR NIH HHS / United States |