Serotonergic neurosecretory synapse targeting is controlled by netrin-releasing guidepost neurons in Caenorhabditis elegans.
Enviado por Daniel Alfonso Colón-Ramos el
Título | Serotonergic neurosecretory synapse targeting is controlled by netrin-releasing guidepost neurons in Caenorhabditis elegans. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Autores | Nelson, JC, Colón-Ramos, DA |
Journal | J Neurosci |
Volume | 33 |
Issue | 4 |
Pagination | 1366-76 |
Date Published | 2013 Jan 23 |
ISSN | 1529-2401 |
Palabras clave | Animals, Animals, Genetically Modified, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cell Communication, Image Processing, Computer-Assisted, Microscopy, Confocal, Nerve Tissue Proteins, Neurons, Serotonergic Neurons, Synapses, Synaptic Transmission |
Abstract | Neurosecretory release sites lack distinct postsynaptic partners, yet target to specific circuits. This targeting specificity regulates local release of neurotransmitters and modulation of adjacent circuits. How neurosecretory release sites target to specific regions is not understood. Here we identify a molecular mechanism that governs the spatial specificity of extrasynaptic neurosecretory terminal (ENT) formation in the serotonergic neurosecretory-motor (NSM) neurons of Caenorhabditis elegans. We show that postembryonic arborization and neurosecretory terminal targeting of the C. elegans NSM neuron is dependent on the Netrin receptor UNC-40/DCC. We observe that UNC-40 localizes to specific neurosecretory terminals at the time of axon arbor formation. This localization is dependent on UNC-6/Netrin, which is expressed by nerve ring neurons that act as guideposts to instruct local arbor and release site formation. We find that both UNC-34/Enabled and MIG-10/Lamellipodin are required downstream of UNC-40 to link the sites of ENT formation to nascent axon arbor extensions. Our findings provide a molecular link between release site development and axon arborization and introduce a novel mechanism that governs the spatial specificity of serotonergic ENTs in vivo. |
DOI | 10.1523/JNEUROSCI.3471-12.2012 |
Alternate Journal | J. Neurosci. |
PubMed ID | 23345213 |
PubMed Central ID | PMC3584569 |
Grant List | 5 T32 NS 41228 / NS / NINDS NIH HHS / United States R00 NS057931 / NS / NINDS NIH HHS / United States R00 NS057931 / NS / NINDS NIH HHS / United States R01 NS076558 / NS / NINDS NIH HHS / United States R01 NS076558 / NS / NINDS NIH HHS / United States |