Type 1 and type 2 cytokine regulation of macrophage endocytosis: differential activation by IL-4/IL-13 as opposed to IFN-gamma or IL-10.
Enviado por Luis Montaner el
Título | Type 1 and type 2 cytokine regulation of macrophage endocytosis: differential activation by IL-4/IL-13 as opposed to IFN-gamma or IL-10. |
Publication Type | Journal Article |
Year of Publication | 1999 |
Autores | Montaner, LJ, da Silva, RP, Sun, J, Sutterwala, S, Hollinshead, M, Vaux, D, Gordon, S |
Journal | J Immunol |
Volume | 162 |
Issue | 8 |
Pagination | 4606-13 |
Date Published | 1999 Apr 15 |
ISSN | 0022-1767 |
Palabras clave | Actins, Cell Nucleus, Endocytosis, Endosomes, Gold Colloid, Golgi Apparatus, Horseradish Peroxidase, Humans, Interferon-gamma, Interleukin-10, Interleukin-13, Interleukin-4, Lectins, C-Type, Lysosomes, Macrophage Activation, Macrophages, Mannose-Binding Lectins, Phosphatidylinositol 3-Kinases, Receptors, Cell Surface, Th1 Cells, Th2 Cells |
Abstract | Cytokine regulation of endocytic activity in primary human macrophages was studied to define ultrastructural changes and mechanisms of pinocytic regulation associated with cytokines secreted by activated T cells. The effects of IFN-gamma (type 1) and IL-4/IL-13 and IL-10 (type 2) cytokines on fluid phase and mannose receptor-mediated endocytosis were assessed by horseradish peroxidase and colloidal gold-BSA uptake and computer-assisted morphometric analysis. IL-4 and IL-13 enhanced fluid phase pinocytosis and mannose receptor-mediated uptake by activation of phosphatidylinositol 3-kinase. Inhibition of actin assembly showed that both cytokines exerted actin-dependent and -independent effects. Ultrastructurally, IL-4 and IL-13 increased tubular vesicle formation underneath the plasma membrane and at pericentriolar sites, concurrent with decreased particle sorting to lysosomes. By contrast, IL-10 or IFN-gamma decreased both fluid phase pinocytosis and mannose receptor-mediated uptake. IFN-gamma stimulated increased particle sorting to perinuclear lysosomes, while IL-10 decreased this activity. In summary, our data document differential effects on macrophage endocytic functions by type 1 or type 2 cytokines associated with induction and effector pathways in immunity. |
Alternate Journal | J. Immunol. |
PubMed ID | 10202000 |
Grant List | AI40379 / AI / NIAID NIH HHS / United States AI43206 / AI / NIAID NIH HHS / United States AI44304 / AI / NIAID NIH HHS / United States / / Wellcome Trust / United Kingdom |