We describe two patients with acute diffuse external otitis (swimmer's otitis) acquired in the Mediterranean shore, with Vibrio alginolyticus recovered from ear fluid. We describe the biochemical profile and sensitivity pattern (MIC) of both strains, comparing it to previously published data. A literature review was also performed, in which we found evidence for increasing concern of V. alginolyticus as an human pathogen. Also there is a need for considering halophilic vibrio as potential pathogens, specially if there is also epidemiologic support.