Zinc oxide nanorods modified indium tin oxide surface for amperometric urea biosensor
Enviado por Jaime E Ramirez-Vick el
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Título | Zinc oxide nanorods modified indium tin oxide surface for amperometric urea biosensor |
Publication Type | Journal Article |
Year of Publication | 2011 |
Autores | Palomera, N, Balaguera, M, Arya, SK, Hernandez, S, Tomar, MS, Ramirez-Vick, JE, Singh, SP |
Journal | J Nanosci NanotechnolJ Nanosci NanotechnolJ Nanosci Nanotechnol |
Volume | 11 |
Pagination | 6683-9 |
Date Published | Aug |
Type of Article | Research Support, Non-U.S. Gov't |
ISBN Number | 1533-4880 (Print)<br/>1533-4880 (Linking) |
Accession Number | 22103068 |
Abstract | ZnO nanorods (ZnONR) grown onto indium-tin-oxide (ITO) coated glass surface using zinc nitrate hexahydrate/hexamethylenetetramine (HMT) in aqueous phase has been utilized for urea biosensor. Urease (Urs) was immobilized onto ZnONR/ITO at physiological pH via electrostatic interactions between Urs and ZnO to fabricate Urs/ZnONR/ITO bioelectrode. ZnONR/ITO electrode has been characterized using XRD, FE-SEM techniques and Urs/ZnONR/ITO bioelectrode using electrochemistry. The XRD and FE-SEM measurements confirm the formation of ZnO nanorods in wurtzite structure. Cyclic voltammetric and amperometric measurements on the Urs/ZnONR/ITO biolectrode for urea concentrations in the range of 1-20 mM reveal 0.4 microA mM(-1) sensitivity, with a response time of 3 seconds, and a detection limit of 0.13 mM. The Michaelis-Menten constant (Km) was calculated to be 9.09 mM. Results indicate that ZnO nanorods provide suitable microenvironment for urease immobilization and can be utilized in biosensor design and other biological applications. |
Short Title | Journal of nanoscience and nanotechnologyJournal of nanoscience and nanotechnology |
Alternate Journal | Journal of nanoscience and nanotechnology |