Welcome to CienciaPR, an expert and resource network for all who are interested in science and Puerto Rico.
Lab techniques: Maintaining human cell cultures (including HEK, glial and monocytic cells), bacterial cells and insect cells (sf9), use of aseptic techniques, expression cloning, RT-PCR, PCR, site-specific mutagenesis, electrophoresis, nucleic acid/protein isolation (small and large scale) and quantitation, Western Blot, transient transfection of mammalian cells, insect cells transfection with recombinant bacmid DNA, protein expression, extraction and purification, electrophysiology patch clamp technique, chromatography, isothermal titration calorimetry, SAXS, crystallization techniques, x-ray crystallography and molecular modeling, synchrotron beamline data collection, enzymatic work using radioisotopes, site-specific spin labeling for EPR experiments, RNA sample preparation for microarrays, use of MS for protein analysisLab instruments: Size exclusion, hydrophobic interaction and cation/anion exchange chromatography including HPLC and FPLC/AKTA, crystallization robots, crystal automounter robots at synchrotron beamline stations, patch-clamp electrophysiology instrument, UV/vis spectrophotometer, CEDEX, Invitrogen iBlot and Benchpro, Thermal Cyclers, ITC instrumentsComputer: Word, Excel, PowerPoint, Endnote, Acrobat, Photoshop, Linux, PHASER for molecular replacement, Vector NTI, Sequencher, DNAStar
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