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My name is Carolina and I am a first year doctoral student at Brown University in their molecular biology, cell biology, and biochemistry (MCB) program. I was born in Bogota, Colombia and immigrated to the US when I was four years old with my parents. I've lived in New York City for pretty much all my life although my scholastic career has taken me to Massachusetts, Maryland, and now Rhode Island. I entered college with the intention of becoming a visual arts and dance double major, however, after my first chemistry class I realized that science was certainly where I belong. While I spend most of my days now in class and in lab, I still enjoying dancing, painting, and designing buildings.
I am in my first rotation so the project I am currently working on might not be my thesis project. Currently, I am investigating the regulation of Lck kinase by phosphatases by performing a BioID screen. The project involves construction of a c terminal BirA fusion protein and characterization of in vivo biotinylation of substrate proteins by mass spectrometry. My task is to develop an optimized protocol for capture of the biotinylated peptides followed by LCMS.
More generally, I am interested in protein engineering. I am stimulated by the idea of tinkering with proteins and conferring optimized function or a totally different function! It's a highly interdisciplinary and translatable field.
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