The effect of integrin-specific bioactive coatings on tissue healing and implant osseointegration.
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Title | The effect of integrin-specific bioactive coatings on tissue healing and implant osseointegration. |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Petrie, TA, Raynor, JE, Reyes, CD, Burns, KL, Collard, DM, García, AJ |
Journal | Biomaterials |
Volume | 29 |
Issue | 19 |
Pagination | 2849-57 |
Date Published | 2008 Jul |
ISSN | 0142-9612 |
Abstract | Implant osseointegration, defined as bone apposition and functional fixation, is a requisite for clinical success in orthopaedic and dental applications, many of which are restricted by implant loosening. Modification of implants to present bioactive motifs such as the RGD cell-adhesive sequence from fibronectin (FN) represents a promising approach in regenerative medicine. However, these biomimetic strategies have yielded only marginal enhancements in tissue healing in vivo. In this study, clinical-grade titanium implants were grafted with a non-fouling oligo(ethylene glycol)-substituted polymer coating functionalized with controlled densities of ligands of varying specificity for target integrin receptors. Biomaterials presenting the alpha5beta1-integrin-specific FN fragment FNIII 7-10 enhanced osteoblastic differentiation in bone marrow stromal cells compared to unmodified titanium and RGD-presenting surfaces. Importantly, FNIII 7-10-functionalized titanium significantly improved functional implant osseointegration compared to RGD-functionalized and unmodified titanium in vivo. This study demonstrates that bioactive coatings that promote integrin binding specificity regulate marrow-derived progenitor osteoblastic differentiation and enhance healing responses and functional integration of biomedical implants. This work identifies an innovative strategy for the rational design of biomaterials for regenerative medicine. |
DOI | 10.1016/j.biomaterials.2008.03.036 |
Alternate Journal | Biomaterials |
PubMed ID | 18406458 |
PubMed Central ID | PMC2397448 |
Grant List | R01 EB-004496 / EB / NIBIB NIH HHS / United States R01 EB004496 / EB / NIBIB NIH HHS / United States R01 EB004496-04 / EB / NIBIB NIH HHS / United States |