Neuromodulatory actions of substance P on the muscarinic receptors of the vas deferens of the rat.
Submitted by Walter Silva on
Title | Neuromodulatory actions of substance P on the muscarinic receptors of the vas deferens of the rat. |
Publication Type | Journal Article |
Year of Publication | 1988 |
Authors | Silva, WI, Miranda, HF, Wolstenholme, WW, Cuevas, N, Ucros, A |
Journal | Neuropeptides |
Volume | 11 |
Issue | 4 |
Pagination | 153-7 |
Date Published | 1988 May-Jun |
ISSN | 0143-4179 |
Keywords | Animals, Atropine, In Vitro Techniques, Male, Muscle, Smooth, N-Methylscopolamine, Neostigmine, Pirenzepine, Rats, Rats, Inbred Strains, Receptors, Muscarinic, Receptors, Neurokinin-1, Receptors, Neurotransmitter, Scopolamine Derivatives, Substance P, Vas Deferens |
Abstract | The response of post-synaptic neurokinin receptors to SP were not changed by pirenzepine or N-methyl-scopolamine. Atropine led to a slight increase in the EC50 of SP for its post-synaptic neurokinin (NK-A) receptor. In the presence of neostigmine no changes in the Emax and EC50 values of SP for its post- and pre-synaptic receptor site were observed. Only the muscarinic receptor site were observed. Only the muscarinic receptor antagonists, atropine and NMS, elicited statistically significant increases in the Emax of SP at its presynaptic receptor (NK-A). Addition of 7.4-740 nM SP resulted in a decrease in the EC50 and Emax values of ACh for its post-synaptic muscarinic receptor (M1). Conversely, 740 nM SP produced an increase in the EC50 and Emax values of ACh at its pre-synaptic muscarinic receptor (M2). Concentrations of 7.4 and 74 nM SP did not produce statistically significant changes in the Emax of ACh for its pre-synaptic M2 receptor. |
Alternate Journal | Neuropeptides |
PubMed ID | 2458536 |
Grant List | 1-G-12-RR03035-01A / RR / NCRR NIH HHS / United States |