Interaction of sulphate and chloride with cobalt(II)-carbonic anhydrase.
Enviado por Magaly Martinez-Ferrer el
Título | Interaction of sulphate and chloride with cobalt(II)-carbonic anhydrase. |
Publication Type | Journal Article |
Year of Publication | 1990 |
Autores | Moratal, JM, Donaire, A, Salgado, J, Martinez-Ferrer, MJ |
Journal | J Inorg Biochem |
Volume | 40 |
Issue | 3 |
Pagination | 245-53 |
Date Published | 1990 Nov |
ISSN | 0162-0134 |
Palabras clave | Carbonic Anhydrase Inhibitors, Carbonic Anhydrases, Chlorides, Cobalt, Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Osmolar Concentration, Spectrophotometry, Sulfates, Temperature |
Abstract | The interaction between Cobalt(II)-Bovine Carbonic Anhydrase II and the inhibitors sulphate and chloride have been investigated through 1H NMR and electronic absorption spectroscopies. Both inhibitors bind to the metal ion forming a 1:1 adduct and the corresponding affinity constants have been determined. These inhibitors interact weakly with CoBCA II and this interaction only occurs at low pH values. The T1 values of the meta-like protons of the coordinated histidines have been measured. The coordination number of the metal ion in the adducts is discussed on the basis of temperature dependence of the isotropic shifts, T1, and molar absorbance values. |
Alternate Journal | J. Inorg. Biochem. |
PubMed ID | 2127282 |