Cystatin B associates with signal transducer and activator of transcription 1 in monocyte-derived and placental macrophages.
Enviado por Loyda Milagros Melendez, Ph.D. el
Título | Cystatin B associates with signal transducer and activator of transcription 1 in monocyte-derived and placental macrophages. |
Publication Type | Journal Article |
Year of Publication | 2009 |
Autores | Luciano-Montalvo, C, Meléndez, LM |
Journal | Placenta |
Volume | 30 |
Issue | 5 |
Pagination | 464-7 |
Date Published | 2009 May |
ISSN | 1532-3102 |
Palabras clave | Cystatin B, Female, HIV Infections, HIV-1, Humans, Macrophages, Phosphorylation, Placenta, Pregnancy, STAT1 Transcription Factor, Tyrosine |
Abstract | Cystatin (CSTB, also known as stefin B), a cysteine protease inhibitor, recently was found to be down-regulated in the proteome of uninfected and HIV-1-infected placental macrophages (PMs) and associated with restricted HIV-1 replication in PM but not in monocyte-derived macrophages (MDMs). We investigated CSTB interactions with signal transducer and activator of transcription 1 (STAT-1) by immunoprecipitation studies because this molecule is known to activate HIV-1 replication. Since both CSTB and STAT-1 are related to HIV-1 replication, we hypothesized that these proteins could be interacting. We applied immunoprecipitation assays to determine STAT-1-CSTB interaction in uninfected and HIV-1-infected PM as compared with MDM. We found that CSTB associates with STAT-1 in PM and MDM. Further analyses indicated that the levels of STAT-1 tyrosine phosphorylation were higher in PM than MDM. High levels of tyrosine phosphorylation previously have been associated with HIV-1 inhibitory activity. This is the first report to demonstrate that cystatin B interacts with STAT-1 and that the levels of STAT-1 tyrosine phosphorylation (but not serine phosphorylation) between uninfected and HIV-infected PM and MDM are differentially regulated. |
DOI | 10.1016/j.placenta.2009.03.003 |
Alternate Journal | Placenta |
PubMed ID | 19342095 |
PubMed Central ID | PMC2683624 |
Grant List | 1 U54NS430 / NS / NINDS NIH HHS / United States G12 RR003051 / RR / NCRR NIH HHS / United States G12 RR003051-226707 / RR / NCRR NIH HHS / United States G12RR03051 / RR / NCRR NIH HHS / United States GM61838 / GM / NIGMS NIH HHS / United States P20 RR011126 / RR / NCRR NIH HHS / United States P20 RR011126-126414 / RR / NCRR NIH HHS / United States P20RR11126 / RR / NCRR NIH HHS / United States S06 GM008224 / GM / NIGMS NIH HHS / United States S06 GM008224-19 / GM / NIGMS NIH HHS / United States S06GMO822 / / PHS HHS / United States U54 NS043011 / NS / NINDS NIH HHS / United States U54 NS043011-01 / NS / NINDS NIH HHS / United States |