Distinct modes of regulated receptor insertion to the somatodendritic plasma membrane.
Enviado por Guillermo Yudowski el
Título | Distinct modes of regulated receptor insertion to the somatodendritic plasma membrane. |
Publication Type | Journal Article |
Year of Publication | 2006 |
Autores | Yudowski, GA, Puthenveedu, MA, von Zastrow, M |
Journal | Nat Neurosci |
Volume | 9 |
Issue | 5 |
Pagination | 622-7 |
Date Published | 2006 May |
ISSN | 1097-6256 |
Palabras clave | Adrenergic beta-Agonists, Animals, Carbazoles, Cell Membrane, Cells, Cultured, Diagnostic Imaging, Drug Interactions, Embryo, Mammalian, Enzyme Inhibitors, Green Fluorescent Proteins, Hippocampus, Indoles, Isoproterenol, Microscopy, Video, Microtubule-Associated Proteins, Neurons, Pyrroles, Rats, Receptors, Adrenergic, beta-2, Time Factors, Transfection |
Abstract | Many neural signaling receptors are regulated by endocytosis, but little is known about receptor insertion into the plasma membrane. Time-lapse imaging of the beta2 adrenergic receptor expressed in cultured rat hippocampal neurons, using pH-sensitive green fluorescent protein tagging and total internal reflection fluorescence microscopy, resolved distinct vesicular fusion events mediating receptor insertion into the somatodendritic plasma membrane. A 'transient' insertion mode resulted in rapid lateral dispersion of receptors immediately after insertion. A 'persistent' insertion mode resulted in the retention of inserted receptors in surface-accessible domains, which were relatively immobile for a prolonged 'wait' period before dispersing laterally. Distinct insertion modes were oppositely regulated by receptor activation and by mechanisms differing in their dependence on the signaling effector cyclic AMP-dependent protein kinase. These results reveal a new mechanism for homeostatic regulation of postsynaptic signaling and a 'kiss-and-wait' mode of regulated membrane protein insertion in neurons. |
DOI | 10.1038/nn1679 |
Alternate Journal | Nat. Neurosci. |
PubMed ID | 16604070 |