Effects of Valeriana officinalis extracts on [3H]flunitrazepam binding, synaptosomal [3H]GABA uptake, and hippocampal [3H]GABA release.
Enviado por Jose G Ortiz-Roque el
Título | Effects of Valeriana officinalis extracts on [3H]flunitrazepam binding, synaptosomal [3H]GABA uptake, and hippocampal [3H]GABA release. |
Publication Type | Journal Article |
Year of Publication | 1999 |
Autores | Ortiz, JG, Nieves-Natal, J, Chavez, P |
Journal | Neurochem Res |
Volume | 24 |
Issue | 11 |
Pagination | 1373-8 |
Date Published | 1999 Nov |
ISSN | 0364-3190 |
Palabras clave | Animals, Drug Synergism, Female, Flunitrazepam, GABA Modulators, gamma-Aminobutyric Acid, Hippocampus, Medicine, Traditional, Mice, Plant Extracts, Plants, Medicinal, Potassium, Rats, Rats, Sprague-Dawley, Synaptosomes, Tritium, Veratridine |
Abstract | Extracts of Valeriana officinalis have been used in folkloric medicine for its sedative, hypnotic, tranquilizer and anticonvulsant effects, and may interact with gamma-aminobutyric acid (GABA) and/or benzodiazepine sites. At low concentrations, valerian extracts enhance [3H]flunitrazepam binding (EC50 4.13 x 10(-10) mg/ml). However, this increased [3H]flunitrazepam binding is replaced by an inhibition at higher concentrations (IC50 of 4.82 x 10(-1) mg/ml). These results are consistent with the presence of at least two different biological activities interacting with [3H]flunitrazepam binding sites. Valerian extracts also potentiate K+ or veratridine-stimulated release of radioactivity from hippocampal slices preloaded with [3H]GABA. Finally, inhibition of synaptosomal [3H]GABA uptake by valerian extracts also displays a biphasic interaction with guvacine. The results confirm that valerian extracts have effects on GABA(A) receptors, but can also interact at other presynaptic components of GABAergic neurons. |
Alternate Journal | Neurochem. Res. |
PubMed ID | 10555777 |