Evidence for the involvement of nucleotide excision repair in the removal of abasic sites in yeast.
Enviado por Carlos A Torres-Ramos el
Título | Evidence for the involvement of nucleotide excision repair in the removal of abasic sites in yeast. |
Publication Type | Journal Article |
Year of Publication | 2000 |
Autores | Torres-Ramos, CA, Johnson, RE, Prakash, L, Prakash, S |
Journal | Mol Cell Biol |
Volume | 20 |
Issue | 10 |
Pagination | 3522-8 |
Date Published | 2000 May |
ISSN | 0270-7306 |
Palabras clave | Alkylating Agents, Carbon-Oxygen Lyases, Deoxyribonuclease IV (Phage T4-Induced), DNA Repair, DNA Repair Enzymes, DNA-(Apurinic or Apyrimidinic Site) Lyase, Endodeoxyribonucleases, Fungal Proteins, Gene Deletion, Methyl Methanesulfonate, Mutagenesis, Mutagens, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins |
Abstract | In eukaryotes, DNA damage induced by ultraviolet light and other agents which distort the helix is removed by nucleotide excision repair (NER) in a fragment approximately 25 to 30 nucleotides long. In humans, a deficiency in NER causes xeroderma pigmentosum (XP), characterized by extreme sensitivity to sunlight and a high incidence of skin cancers. Abasic (AP) sites are formed in DNA as a result of spontaneous base loss and from the action of DNA glycosylases involved in base excision repair. In Saccharomyces cerevisiae, AP sites are removed via the action of two class II AP endonucleases, Apn1 and Apn2. Here, we provide evidence for the involvement of NER in the removal of AP sites and show that NER competes with Apn1 and Apn2 in this repair process. Inactivation of NER in the apn1Delta or apn1Delta apn2Delta strain enhances sensitivity to the monofunctional alkylating agent methyl methanesulfonate and leads to further impairment in the cellular ability to remove AP sites. A deficiency in the repair of AP sites may contribute to the internal cancers and progressive neurodegeneration that occur in XP patients. |
Alternate Journal | Mol. Cell. Biol. |
PubMed ID | 10779341 |
PubMed Central ID | PMC85644 |
Grant List | CA41261 / CA / NCI NIH HHS / United States |