Increased CD34+/KDR+ cells are not associated with carotid artery intima-media thickness progression in chronic HIV-positive subjects.
Enviado por Luis Montaner el
Título | Increased CD34+/KDR+ cells are not associated with carotid artery intima-media thickness progression in chronic HIV-positive subjects. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Autores | Papasavvas, E, Hsue, P, Reynolds, G, Pistilli, M, Hancock, A, Martin, JN, Deeks, SG, Montaner, LJ |
Journal | Antivir Ther |
Volume | 17 |
Issue | 3 |
Pagination | 557-63 |
Date Published | 2012 |
ISSN | 2040-2058 |
Palabras clave | Antigens, CD34, Atherosclerosis, Carotid Arteries, Carotid Intima-Media Thickness, Chronic Disease, Disease Progression, Endothelial Cells, Endothelium, Vascular, Female, Flow Cytometry, HIV Infections, Humans, Male, Middle Aged, Stem Cells, Tunica Intima, Tunica Media |
Abstract | BACKGROUND: Endothelial progenitor cells (EPCs) are involved in the endothelium repair. Low circulating EPC levels are predictive of cardiovascular events in HIV-negative subjects. The impact of HIV infection on EPCs, and the role of EPCs in HIV-associated cardiovascular disease, is not known. We hypothesized that circulating EPCs would be inversely associated with carotid artery intima-media thickness (c-IMT) changes in HIV-infected subjects. METHODS: EPCs (CD34(+)/KDR(+), CD133(+)/KDR(+) and CD34(+)/CD133(+)/KDR(+)) were defined retrospectively by flow cytometry in cryopreserved peripheral blood mononuclear cells collected longitudinally from 66 chronic HIV-infected subjects and cross-sectionally from 50 at-risk HIV-negative subjects. The HIV-infected subjects participated in the Study of the Consequences of the Protease Inhibitor Era (SCOPE) cohort, were receiving antiretroviral therapy (59/66) and had two sequential measurements of c-IMT 1 year apart. Two distinct groups of HIV-infected subjects were identified a priori: rapid c-IMT progressors (subjects with rapid c-IMT progression, n=13, Δc-IMT>0.2 mm) and slow c-IMT progressors (subjects with slow or no c-IMT progression, n=53, Δc-IMT<0.2 mm). RESULTS: Although cryopreservation reduced sensitivity of detection, EPC frequency in HIV-infected subjects was still significantly higher compared to at-risk HIV-negative subjects (CD34(+)/KDR(+); P=0.01) and correlated positively with CD4(+) T-cell count (CD34(+)/KDR(+), r=0.27; P=0.03). No association was found between the change of EPC frequencies over time (ΔEPC) and Δc-IMT or between EPC frequencies and c-IMT or Δc-IMT. CONCLUSIONS: The lack of an association between EPCs and c-IMT in our cohort does not support HIV-associated reductions in EPC frequency as a cause of accelerated atherosclerosis. |
DOI | 10.3851/IMP2013 |
Alternate Journal | Antivir. Ther. (Lond.) |
PubMed ID | 22300770 |
PubMed Central ID | PMC3362125 |
Grant List | 5K23AI066885 / AI / NIAID NIH HHS / United States 5R01HL095130 / HL / NHLBI NIH HHS / United States AI 065279 / AI / NIAID NIH HHS / United States AI051986 / AI / NIAID NIH HHS / United States AI48398 / AI / NIAID NIH HHS / United States K23 AI066885 / AI / NIAID NIH HHS / United States K24 AI069994 / AI / NIAID NIH HHS / United States K24 AI069994 / AI / NIAID NIH HHS / United States P30 AI027763 / AI / NIAID NIH HHS / United States P30 AI27763 / AI / NIAID NIH HHS / United States P30 MH059037 / MH / NIMH NIH HHS / United States P30 MH062246 / MH / NIMH NIH HHS / United States P30 MH59037 / MH / NIMH NIH HHS / United States P30 MH62246 / MH / NIMH NIH HHS / United States R01 AI048398 / AI / NIAID NIH HHS / United States R01 AI051986 / AI / NIAID NIH HHS / United States R01 HL095130 / HL / NHLBI NIH HHS / United States U01 AI065279 / AI / NIAID NIH HHS / United States UL1 RR024131 / RR / NCRR NIH HHS / United States UL1 RR024131-01 / RR / NCRR NIH HHS / United States |