Characterization and differential expression of a ferritin protein from Fasciola hepatica.
Enviado por Kimberly Cabán-Hernández el
Título | Characterization and differential expression of a ferritin protein from Fasciola hepatica. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Autores | Cabán-Hernández, K, Gaudier, JF, Espino, AM |
Journal | Mol Biochem Parasitol |
Volume | 182 |
Issue | 1-2 |
Pagination | 54-61 |
Date Published | 2012 Mar-Apr |
ISSN | 1872-9428 |
Palabras clave | Amino Acid Sequence, Animals, Antigens, Helminth, Biological Markers, Cloning, Molecular, DNA, Complementary, Escherichia coli, Fasciola hepatica, Fascioliasis, Ferritins, Gene Expression Regulation, Developmental, Helminth Proteins, Immunologic Tests, Iron, Molecular Sequence Data, Phylogeny, Protein Structure, Secondary, Rabbits, Recombinant Proteins, RNA, Messenger, Sequence Alignment |
Abstract | Ferritins are proteins that play a central role in maintaining intracellular iron balance. A cDNA clone of Fasciola hepatica (687 bp long) encoding a putative 228-amino acid polypeptide (FhFtn-1) homologous with ferritins of vertebrates and invertebrates was identified. FhFtn-1 contains a conserved motif of the ferroxidase center typical of vertebrate ferritins. Phylogenetic tree analysis showed that FhFtn-1 clusters with two ferritins of Paragonimus westermani, which suggests a common ancestry for the ferritins of these two trematodes. Recombinant FhFtn-1 protein expressed and purified from an Escherichia coli system showed iron-uptake ability. Moreover, FhFtn-1 showed strong reactivity with sera from rabbits infected with F. hepatica for 2-12 weeks, which suggests that this protein could be a potential antigen for immunodiagnosis of fascioliasis. qPCR analysis demonstrated that FhFtn-1-mRNA is expressed at significantly higher levels in adults and unembryonated eggs than in juveniles or miracidia. These results represent the first characterization of a ferritin protein from the liver fluke F. hepatica. |
DOI | 10.1016/j.molbiopara.2011.12.005 |
Alternate Journal | Mol. Biochem. Parasitol. |
PubMed ID | 22240114 |
PubMed Central ID | PMC3277670 |
Grant List | 1SC1AI096108-01A2 / AI / NIAID NIH HHS / United States 3 U54 RR022762-0351 / RR / NCRR NIH HHS / United States R25GM061838 / GM / NIGMS NIH HHS / United States SC1 AI096108 / AI / NIAID NIH HHS / United States SC1 AI096108-01A2 / AI / NIAID NIH HHS / United States U54 RR022762 / RR / NCRR NIH HHS / United States |