Comparison of the convergent receptor utilization of a retargeted feline leukemia virus envelope with a naturally-occurring porcine endogenous retrovirus A.
Enviado por Leonardo Valdivieso-Torres el
Título | Comparison of the convergent receptor utilization of a retargeted feline leukemia virus envelope with a naturally-occurring porcine endogenous retrovirus A. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Autores | Mazari, PM, Argaw, T, Valdivieso, L, Zhang, X, Marcucci, KT, Salomon, DR, Wilson, CA, Roth, MJ |
Journal | Virology |
Volume | 427 |
Issue | 2 |
Pagination | 118-26 |
Date Published | 2012 Jun 5 |
ISSN | 1096-0341 |
Palabras clave | Amino Acid Sequence, Animals, Cats, Cell Line, Endogenous Retroviruses, Gene Expression Regulation, Viral, Humans, Leukemia Virus, Feline, Mutation, Protein Binding, Receptors, Cell Surface, Recombinant Proteins, Swine, Viral Envelope Proteins, Viral Tropism, Virus Internalization |
Abstract | In vitro screening of randomized FeLV Envelope libraries identified the CP isolate, which enters cells through HuPAR-1, one of two human receptors utilized by porcine endogenous retrovirus-A (PERV-A), a distantly related gammaretrovirus. The CP and PERV-A Envs however, share little amino acid homology. Their receptor utilization was examined to define the common receptor usage of these disparate viral Envs. We demonstrate that the receptor usage of CP extends to HuPAR-2 but not to the porcine receptor PoPAR, the cognate receptor for PERV-A. Reciprocal interference between virus expressing CP and PERV-A Envs was observed on human cells. Amino acid residues localized to within the putative second extracellular loop (ECL-2) of PAR-1 and PAR-2 are found to be critical for CP envelope function. Through a panel of receptor chimeras and point mutations, this area was also found to be responsible for the differential usage of the PoPAR receptor between CP and PERV-A. |
DOI | 10.1016/j.virol.2012.02.012 |
Alternate Journal | Virology |
PubMed ID | 22405627 |
PubMed Central ID | PMC3572736 |
Grant List | R01 AI052349 / AI / NIAID NIH HHS / United States R01 CA049932-17 / CA / NCI NIH HHS / United States R01 CA049932-18 / CA / NCI NIH HHS / United States R01 CA049932-19 / CA / NCI NIH HHS / United States R01 CA049932-19S1 / CA / NCI NIH HHS / United States R01 CA49932 / CA / NCI NIH HHS / United States T32 GM008360 / GM / NIGMS NIH HHS / United States |