Prevalence and distribution of introns in non-ribosomal protein genes of yeast.
Enviado por Jose R Rodriguez-Medina el
Título | Prevalence and distribution of introns in non-ribosomal protein genes of yeast. |
Publication Type | Journal Article |
Year of Publication | 1994 |
Autores | Rodriguez-Medina, JR, Rymond, BC |
Journal | Mol Gen Genet |
Volume | 243 |
Issue | 5 |
Pagination | 532-9 |
Date Published | 1994 Jun 3 |
ISSN | 0026-8925 |
Palabras clave | Base Sequence, Databases, Factual, DEAD-box RNA Helicases, DNA Primers, Electrophoresis, Gel, Two-Dimensional, Fungal Proteins, Genome, Fungal, Introns, Molecular Sequence Data, Multigene Family, Poly A, Prevalence, Ribosomal Proteins, RNA Precursors, RNA Splicing, RNA, Messenger, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Spliceosomes |
Abstract | Relatively few genes in the yeast Saccharomyces cerevisiae are known to contain intervening sequences. As a group, yeast ribosomal protein genes exhibit a higher prevalence of introns when compared to non-ribosomal protein genes. In an effort to quantify this bias we have estimated the prevalence of intron sequences among non-ribosomal protein genes by assessing the number of prp2-sensitive mRNAs in an in vitro translation assay. These results, combined with an updated survey of the GenBank DNA database, support an estimate of 2.5% for intron-containing non-ribosomal protein genes. Furthermore, our observations reveal an intriguing distinction between the distributions of ribosomal protein and non-ribosomal protein intron lengths, suggestive of distinct, gene class-specific evolutionary pressures. |
Alternate Journal | Mol. Gen. Genet. |
PubMed ID | 7911556 |
Grant List | GM08224 / GM / NIGMS NIH HHS / United States GM42476 / GM / NIGMS NIH HHS / United States RR03051 / RR / NCRR NIH HHS / United States |