Sequence analysis of a Fasciola hepatica glutathione S-transferase cDNA clone.
Enviado por Jose R Rodriguez-Medina el
Título | Sequence analysis of a Fasciola hepatica glutathione S-transferase cDNA clone. |
Publication Type | Journal Article |
Year of Publication | 1993 |
Autores | Muro, A, Rodriguez-Medina, JR, Hillyer, GV |
Journal | Am J Trop Med Hyg |
Volume | 48 |
Issue | 3 |
Pagination | 457-63 |
Date Published | 1993 Mar |
ISSN | 0002-9637 |
Palabras clave | Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA, Electrophoresis, Agar Gel, Epitopes, Fasciola hepatica, Glutathione Transferase, Molecular Sequence Data, Polymerase Chain Reaction, Schistosoma japonicum, Schistosoma mansoni, Sequence Homology, Amino Acid |
Abstract | A lambda gt11 Fasciola hepatica cDNA library was previously constructed from poly(A)+ RNA extracted from adult worms. A cDNA encoding F. hepatica glutathione S-transferase (FhGST) (GenBank Accession Number M93434) was cloned by screening the library with a rabbit anti-FhGST antiserum. The FhGSTs are suspected to be early developmentally expressed-proteins that act as potent immunogens and may be useful as candidate vaccines against the parasite. The cDNA was sequenced and contains 627 translated bases encoding a 24,211-Dalton polypeptide with 209 amino acids and an isoelectric point (pI) of 5.324. This polypeptide has significant homology with 26-kD GSTs from Schistosoma mansoni (57.42%) and S. japonicum (57.14%). In addition, we have identified the predicted T cell epitopes in the FhGST protein molecule. |
Alternate Journal | Am. J. Trop. Med. Hyg. |
PubMed ID | 7682383 |
Grant List | RR-03051 / RR / NCRR NIH HHS / United States |