IL-13 acts on macrophages to block the completion of reverse transcription, inhibit virus production, and reduce virus infectivity.
Enviado por Luis Montaner el
Título | IL-13 acts on macrophages to block the completion of reverse transcription, inhibit virus production, and reduce virus infectivity. |
Publication Type | Journal Article |
Year of Publication | 1997 |
Autores | Montaner, LJ, Bailer, RT, Gordon, S |
Journal | J Leukoc Biol |
Volume | 62 |
Issue | 1 |
Pagination | 126-32 |
Date Published | 1997 Jul |
ISSN | 0741-5400 |
Palabras clave | Cell Differentiation, Cells, Cultured, DNA, Viral, Genes, gag, HIV Long Terminal Repeat, HIV Reverse Transcriptase, HIV-1, Humans, Interleukin-13, Interleukin-4, Kinetics, Macrophages, Monocytes, Virus Replication |
Abstract | An understanding of the immune suppression of HIV-1 replication in macrophages continues to be a major goal of AIDS research due to the central role this cell type has in AIDS pathogenesis. We have previously discussed the potential clinical benefits of the anti-inflammatory cytokine interleukin-13 (IL-13), which, unlike IL-4 or IL-10, had limited effects on T cell functions. In this report are extend our observations on the effects of IL-13 on HIV-1 replication in monocyte-derived macrophages (MDM) and show redundancy with IL-4, IL-13 or IL-4 have similar effects on HIV-1 replication in MDM when added at different times after infection, with the ability to decrease infection virus release when added for up to 7 days after infection. Removal of IL-13 from MDM revealed a reduction of infection by 16- to 81-fold based on the absence of viral re-emergence from lower multiplicity of infection (m.o.i.). The reduction of HIV-1 infectivity in MDM caused by IL-13 was further characterized by studies on the formation of viral DNA over a range of m.o.i. IL-13 increased the formation of LTR DNA at the lowest m.o.i. of 0.007 while concurrently inhibiting the formation of gag DNA, a later reverse transcription product, at the highest m.o.i. tested, 0.62. Overall, our data indicate that IL-13 can act on macrophages before and after HIV-1 infection by blocking the completion of reverse transcription, decreasing virus production, and reducing the infectivity of the progeny virions. |
Alternate Journal | J. Leukoc. Biol. |
PubMed ID | 9226003 |
Grant List | AI40379 / AI / NIAID NIH HHS / United States |